For nonpolar extraction, choose RP-18, RP-18E (Endcapped), or CN (Cyano) columns.

Typical sample matrix: aqueous buffer solution. Typical sample substances: aromatic ring systems and compounds with alkyl chains. Typical elution solvent: acetonitrile, methanol, and ethyl acetate. Endcapped cartridges have a bonded phase that is reacted with trimethylchlorosilane to cover silanols not previously reacted with the initial bonding reagent. For polar extraction, choose CN (Cyano) or Si (Silica Gel) columns. Typical sample matrix: hexane, oils, and chlorinated hydrocarbons. Typical sample substances: hydroxyl groups, amines, and compounds with hetero-atoms (S, N, O). Typical elution solvent: methanol and 2-propanol.

For nonpolar extraction on a polymer phase, choose EN columns. Typical sample matrix: drinking water, ground water, surface water, and body fluids. Typical sample substances: polar contaminants, pesticides, phenols, explosives, anilines, and pharmaceuticals. Typical elution solvent: ethyl acetate, methanol, acetonitrile, and methanol (1:1).

For anion exchange extraction, choose NH₂ (weak) columns. Typical sample matrix: methanolic/aqueous buffer with low ionic strength, 2 pH units over pK value of the sample substance. Typical sample substances: anions, carboxylic acids, sulfonic acids, and phosphates. Typical elution solvent: aqueous buffer of high ionic strength (0.1 mol/L), 2 pH units over pK value of the sample substance.

For cation exchange extraction choose SCX (strong) columns. Typical sample matrix: methanolic/aqueous buffer with low ionic strength, 2 pH units under pK value of the sample substance. Typical sample substances: cations, amines, and pyrimidines. Typical elution solvent: aqueous buffer of high ionic strength (0.1 mol/L), 2 pH units over pK value of the sample substance.

For mixed mode extraction, choose TSC columns. Typical sample matrix: body fluids. Typical sample substances: cationic and neutral analytes. Typical elution solvent: chloroform-acetone, NH₃-ethyl-acetate, and NH₃-methanol.