Sulfo-SBED is a trifunctional crosslinking reagent containing a biotin, a sulfonated N-hydroxysuccinimide (Sulfo-NHS) active ester and a photoactivatable aryl azide

• Eliminates the need to radiolabel
• Segregated biotin nucleus
• Ideal for protein:protein interaction studies using the label transfer method

This allows for easy purification and detection of interacting proteins. This ready-to-use kit combines the most commonly used components in label transfer applications to save preparation time. Components are supplied in single-use packaging to eliminate waste and provide a longer shelf life. In addition, extensive guidelines are provided with the kit to aid in critical aspects of reagent use. For additional purification, add the protein:protein interaction reagent to the protocol. It features a photoreactive aryl nitrene group, a sulfo-NHS-ester group, and a biotin group for increased flexibility in any protocol. The cleavable disulfide bond allows molecules to be easily detached from each other after conjugates are recovered.

Sulfo-SBED Biotin Label Transfer Reagent is a multifunctional reagent for labeling a purified protein and then covalently transferring the attached biotin tag onto specific interactors of that protein.