A complete system for detection of biotin-labeled nucleic acids for various blotting applications including Northern/Southern blots, ribonuclease protection assays (RPAs), electrophoretic mobility shift assays (EMSAs or gel shifts).

• Fast and sensitive
• Versatile kit protocol (included) is applicable to any biotin-based nucleic acid detection system: Northerns, Southerns, EMSA, RPAs and colony lifts
• Kit contains only the critical detection components for those who already have biotin-labeled probes and an optimized hybridization method developed

It is designed for those researchers who already have biotin-labeled probes and an optimized hybridization method developed to allow for quick and sensitive detection results in a wide variety of protocol applications.

The system uses an enhanced luminol substrate for horseradish peroxidase (HRP) with optimized blocking and wash steps that together produce sensitivity equivalent to radioactive (³²P) systems and provide a clean, consistent background required for publication-quality data.