The NEXTFLEX™ small RNA-sequence kit V4 enables labs to reduce bias in their Illumina® small RNA-sequence workflows while leveraging a completely gel-free protocol, even with low input samples.
- Completely gel-free protocol from purified miRNA or 1 ng of total RNA
- Automated on the Sciclone® G3 NGSx, Sciclone G3 NGSx iQ™, and the Zephyr® G3 NGS workstations
- Patent pending technology optimized for challenging samples such as plasma or serum, including exosomes
- Exceptional miRNA discovery
- Ready to sequence libraries in ~6 hours
- Multiplexing of up to 384 samples
- An automation protocol is available
The NEXTFLEX™ small RNA-sequence kit V4 uses patent-pending technology to provide a completely gel-free small RNA library preparation solution for Illumina sequencing platforms. Our approach to reducing bias involves optimization of the ligation reaction and the use of dimer reduction oligonucleotides. These changes result in decreased bias in comparison to standard protocols. NEXTFLEX™ small RNA-sequence kit V4 allows preparation of libraries ready for sequencing with a simplified workflow in approximately 6 hours, with as little input as 1 ng of total RNA. Libraries prepared with this kit have a higher proportion of mapping reads and unique miRNAs than leading competitors. This convenient gel-free workflow incorporated in the NEXTFLEX™ small RNA-sequence kit V4 enables the construction of libraries from exoRNA. Using this workflow, researchers can achieve a high number of reads aligning to mature miRNA and low adapter dimers, even with the very low miRNA inputs characteristic of exosome samples.