The NEXTFLEX® Rapid XP V2 DNA-sequence kit offers a simplified DNA-seq library prep workflow for Illumina® platforms. Libraries produced using this kit are normalized during library construction.

• Fully automated normalization and fragmentation
• Enzymatic fragmentation with end-repair and A-tailing in one reaction
• Highly efficient ligation with low adapter dimer formation
• Efficient: Automated on the Sciclone®  and Zephyr®  NGS workstations for mid-high throughput automation and now on the BioQule™ NGS System for low throughput labs

The NEXTFLEX® Rapid XP V2 DNA-seq kit includes proprietary NEXTFLEX® normalization beads, which provide a consistent mass and sequencing cluster density for all samples in a library pool. This shortens the time needed for quantification and pooling preparation for sequencing in a high throughput lab by up to 3 hours per 96 samples. The NEXTFLEX  Rapid XP V2 DNA-seq kit offers a streamlined workflow with highly efficient ligation, generating low amount of adapter dimers even at low input concentrations.

The NEXTFLEX Rapid XP V2 DNA-Seq kit is compatible with multiple liquid handlers, including the Sciclone G3 NGSx workstation, Sciclone G3 NGSx iQ™ workstation, and Zephyr NGS workstation. For low throughput automation, the NEXTFLEX  Rapid XP V2 DNA-Seq kit is also now automated on the BioQule™ NGS System which simplifies both library prep and quantitation. This low-cost, benchtop instrument delivers libraries ready to load into your sequencer with only 15 minutes of hands-on time.