For quantitative determination of ammonia and ammonium in biological samples.

• Fast and sensitive
• Linear detection range of 0.005 to 2 mM ammonia
• Convenient and high-throughput
• A single component reagent
• Homogeneous 'mix-incubate-measure' type assay
• Can be readily automated on HTS liquid handling systems for processing thousands of samples per day

AMMONIA (NH₃) or its ion form ammonium (NH₄⁺) is an important source of nitrogen for living systems and is ubiquitously present in the nature. Simple, direct and automation-ready procedures for measuring NH₃ are very desirable. BioAssay Systems’ updated ammonia/ammonium assay is based on an improved o-phthalaldehyde method. This reagent reacts with ammonia/ammonium and forms a fluorescent product. The fluorescence intensity (λex/em = 415/475 nm) is proportional to the ammonia concentration in the sample.