For quantitative determination of glutaminase activity in biological samples.

• Fast and safe
• Assay can be completed within 40 minutes
• Non-radioactive assay
• Sensitive and accurate
• Linear detection range is 0.66 to 500 U/L glutaminase in a 96-well plate assay
• Convenient and high-throughput
• Homogeneous 'mix-incubate-measure' type assay
• Can be readily automated to assay thousands of samples per day

Glutaminase (EC 3.5.1.2) is a key enzyme in the metabolism of glutamine. Glutamine is an important amino acid in the metabolism of cancer cells, and increased glutaminolysis has been linked to cancer. BioAssay systems DGLN-100 kit provides a convenient fluorimetric method to measure glutaminase activity in biological samples.

In this assay, o-phthalaldehyde reacts with liberated ammonia where the increase in fluorescence at λex/em = 415/475 nm is directly proportional to enzyme activity.