PureCube Zn-NTA agarose resins are small agarose resin beads with a diameter of 40 µm. They are used for the purification of active His-tagged proteins from cells and the enrichment of Zinc-Binding proteins like Zinc-fingers.

• Usage: Specific binding and purification of 6× His-tagged proteins, Binding of Zinc-binding proteins
• Specificity: Affinity to His-tagged proteins, Affinity to Zinc-finger proteins
• Binding capacity: >50 mg/ml
• Bead ligand: Zn-NTA
• Bead size: 40 μm
• Filling quantity: Delivered as a 50% suspension
• Metal ion capacity: >15 µeqv Zn²⁺/ml
• pH stability: 2 to 14
• Other stabilities: 100% methanol, 100% ethanol, 8 M urea, 6 M guanidinium hydrochloride, 30% (v/v) acetonitrile

Our year-long experience in manufacturing agarose resin led to the high yield of 50 mg protein per ml resin, which is leading in the market compared to other Zn-NTA suppliers. PureCube Zn-NTA resins are suited for batch spin columns and FPLC. Their small diameter provides them with great mechanical stability.

Required equipment:
-Lysis buffer
-Wash buffer
-Elution buffer
-Ice bath
-Refrigerated centrifuge for 50 ml tube (min 1000×g)
-50 ml centrifuge tube
-Micropipettor and Micropipetting tips
-Disposable gravity flow columns with capped bottom outlet, 2 ml
-pH meter
-End-over-end shaker
-SDS-PAGE buffers, reagents and equipment Optional: Western Blot reagents and equipment