PureCube Maleimide MagBeads are your best option to couple biomolecules that carry thiol (SH) groups.

• Usage: Coupling proteins to active Maleimide Groups
• Specificity: Exposed thiol (SH) groups on biomolecule
• Bead ligand: Chemically active Maleimide groups
• Resulting bond: Irreversible Thioether
• Protein binding capacity: ≥15 mg/ml
• Bead size: 30 μm
• Filling quantity: Delivered as a 25% suspension
• Matrix: 6% cross-linked agarose
• Short term storage: In neutral buffer at 4 °C
• Long term storage: 100 mM sodium hydrogen carbonate, 0.02% sodium azide, pH 7.5 at 4 °C or 20 mM sodium acetate, 20% ethanol, pH 6.5 at 4 °C
• Shelf life: If properly stored - three months upon delivery, unopened
• Chemical Structure: Maleimide structure

The resulting Thioether bond between the Maleimide MagBeads and your biomolecule of interest is stable so that the bead can be used for other applications afterward. Our PureCube Maleimide Activated MagBeads are also available as agarose resin if you prefer this method catalog or as XL MagBeads if you need higher flow rates for your downstream application.