HiCap StrepTactin® Compact Cartridge is a FPLC-ready column that is pre-packed with our PureCube HiCap StrepTactin® agarose resin.

• Usage: Highly specific affinity purification of Strep-(WSHPQFEK)-tagged proteins
• Specificity:Affinity to Strep-tagged (WSHPQFEK) proteins
• Binding capacity: >5 mg/ml
• Bead Ligand: StrepTactin®
• Bead size: 100 μm
• Matrix: 6% cross-linked agarose
• Recommended flow rates: 0.25 to 1 ml/min
• Max. flow rates: 6 ml/min
• Filling quantity: Delivered as a 50% suspension
• Chemical stability: 2% Tween 20, 2% Triton X-100, 2% IGEPAL® 630/Nonidet P40, n-Octyl-beta-D-glucopyranoside, 0,2% n-Nonyl-beta-D-glucopyranoside, 0,35% N-Decyl-beta-D-maltoside, 2% Lauryl-sarcosine, 0, 1% SDS, 0,3% CHAPS, 1M Guanidine HCl, 1mM PMSF, 10% Ethanol, 5M NaCl, 2M (NH4)SO₄, 1M CaCl₂, 25% Glycerol
• Short-term storage: In neutral buffer at 4 °C
• Long-term storage: In neutral buffer with 20% ethanol at 4 °C

PureCube StrepTactin® agarose resin beads consist of 6% cross-linked agarose coupled to the StrepTactin protein.

Required equipment and materials:
-FPLC hardware like e.g. ÄKTA
-Binding Buffer
-Elution Buffer