About this item
PureCube IDA (iminodiacetic acid) agarose resin was developed for IMAC-based protein purification and protein enrichment methods.
- Usage: For coupling of metal ions for corresponding purification and/or enrichment purposes
- Specificity: Varies
- Binding capacity: Depends on the loaded ion
- Purity: Depends on the loaded ion
- Bead ligand: IDA
- Bead size: 40 μm
- Matrix: 7.5% cross-linked agarose
- Filling quantity: Delivered as a 50% suspension
- Recommended FPLC flow rates: 0.5 to 2 ml/min
- Maximal FPLC flow rate: 6 ml/min
- pH stability: 2 to 14
- Other stabilities: 100% methanol, 100% ethanol, 8 M urea, 6 M guanidinium hydrochloride, 30% (v/v) acetonitrile
The beads in this suspension have an average diameter of 40 µm and, thus, excellent mechanical stability. Since the affinity of an IDA-metal-ion-complex depends on the loaded metal ion, we offer IDA agarose resin uncoupled to any metal ion in case we do not offer your desired IDA + metal ion combination. Through our coupling protocol, you can, in turn, attach your desired metal ions to the agarose beads. PureCube IDA resin is also available with a bead diameter of 400 µm for higher FPLC flow rates or as IDA magnetic beads. Furthermore, PureCube IDA agarose is also obtainable pre-packed into FPLC cartridges/columns.