PureCube Fe-NTA compact cartridge is an FPLC-ready column pre-packed with our PureCube Fe-NTA agarose resin.

• Purity: >90% (Determined by SDS-PAGE)
• Number of amino acids: 184
• molecular mass: 21468 Da
• Extinction coefficient (in water) ε280:19940 M⁻¹cm⁻¹
• Buffer: 20 mM TRIS pH 7.4; 100 mM NaCl; 0.5 mM EDTA
• Origin: Human
• Sequence (1-Letter Code): MGSSHHHHHH ENLYFQ GSTFSKLREQ LGPVTQEFWD NLEKETEGLR QEMSKDLEEV AKVQPYLDDF QKKWQEEMEL RQKVEPLGEE MRDRARAHVD ALRTHLAPYS DELRQRLAAR LEALKENGGA RLAEYHAKAT EHLSTLSEKA KPALEDLRQG LLPVLESFKV SFLSALEEYT KKLNTQ
• Affinity tag: N-Terminal His-tag

PureCube Fe-NTA is used for the purification of His-tagged proteins and the enrichment of phosphorylated proteins. The PureCube cartridge/column that is used here is compatible with almost all commonly used FPLC systems like ÄKTA TM. As an alternative, we also offer PureCube Fe-NTA MagBeads, the more common format of Fe-NTA beads for phosphopeptide enrichment.