PureCube Ti-NTA MagBeads/magnetic beads were developed for the enrichment of phosphorylated proteins. They can also be used for the purification of His-tagged proteins as a secondary function.

• Usage: Specific binding and purification of 6× His-tagged proteins, binding of copper-binding proteins
• Specificity: Affinity to His-tagged proteins, affinity to copper-binding
• Binding capacity: >80 mg/ml
• Bead Ligand: Cu-NTA
• Bead size: 40 μm
• Chelator stability: Stable in buffer containing 10 mM DTT and 1 mM EDTA
• Filling quantity: Delivered as a 50% suspension

Required equipment and materials:
- FPLC hardware like e.g. AKT
- Binding buffer
- Elution buffer

The affinity matrix is based on 6% cross-linked agarose that was magnetized. The bead ligand is Ti-NTA and the particle diameter is 30 µm. They are very homogeneous in size, yielding in a high degree of reproducibility. The phosphopeptide enrichment with Cube Biotech MagBeads can be automated.