PureCube maleimide agarose XL resin is your best option to couple biomolecules carrying thiol (SH) groups.

• Usage: Specific binding and purification of 6× His tagged proteins
• Specificity: Affinity to His tagged proteins
• Binding capacity: 20 to 80 mg/ml, depending on bead size
• Bead Ligand: Ni-NTA
• Bead size: 40 μm, 100 µm, or 400 µm
• Chelator stability: Stable in buffer containing 10 mM DTT and 1 mM EDTA
• Filling quantity: Delivered as a 50% suspension

Required equipment:
- Lysis buffer
- Wash buffer
- Elution buffer
- Ice bath
- Refrigerated centrifuge for 50 ml tube (min 10000×g)
- 50 ml centrifuge tube
- Micropipettor and micropipetting tips
- Disposable gravity flow columns with capped bottom outlet, 2 ml
- pH meter
- End-over-end shaker
- SDS-PAGE buffers, reagents, and equipment optional: Western Blot reagents and equipment

The resulting Thioether bond between the maleimide agarose beads and your biomolecule of interest is stable so that the bead can be used for other applications afterward. Our PureCube maleimide activated agarose is also available as magnetic beads in case you prefer this method, or as agarose resin with a smaller bead diameter if your downstream application requires more mechanically stable beads.