Our PureCube phenyl agarose resin is a suspension of purification beads for hydrophobic interaction chromatography.

• Antibody name: Rho-1d4-1
• Cell line/origin: Mouse, hybridoma
• Antibody purification: rProtein A
• Quantity: 200 µg
• Concentration: 4.11 mg/ml (by UV absorbance at 280 nm)
• Physical State: Liquid (sterile filtered)
• Color: Clear
• Buffer: 0.25 M sodium bicarbonate, 0.5 M sodium chloride, pH = 8.3
• Purity: >95% (determined by non-denaturing SDS-PAGE)
• Shipment temperature: Shipped on ice
• Short-term storage: Store vial at 4 °C. Centrifuge product if not completely clear after standing at room temperature and/or heating to 37 °C
• Long-term Storage: Aliquot contents and freeze at –20 °C or below. Avoid repeated freeze-thaw cycles

Hydrophobic Interaction Chromatography (HIC) is a reliable method to separate proteins and other biomolecules based on their hydrophobicity. If you use HIC with your sample for the first time we recommend testing all of our HIC resins before.