The Rho1D4 starters set 2 combines our HighSpec Rho1D4 MagBeads and the Rho1D4 elution peptide.

• Usage: Highly specific affinity purification of Strep-(WSHPQFEK)-tagged proteins
• Specificity: Affinity to Strep-tagged (WSHPQFEK) proteins
• Binding capacity: >5 mg/ml
• Bead ligand: StrepTactin®
• Bead size: 100 μm
• Matrix: 6% cross-linked agarose
• Recommended flow rates: 0.25 to 1 ml/min
• Max. flow rates: 6 ml/min
• Filling quantity: Delivered as a 50% suspension
• Chemical stability: 2% tween 20, 2% triton X-100, 2% IGEPAL® 630/nonidet P40, n-octyl-beta-D-glucopyranoside, 0,2% n-nonyl-beta-D-glucopyranoside, 0,35% N-decyl-beta-D-maltoside, 2% lauryl-sarcosine, 0, 1% SDS, 0,3% CHAPS, 1 M guanidine HCl, 1 mM PMSF, 10% ethanol, 5 M NaCl, 2 M (NH₄)SO₄, 1 M CaCl₂, 25% glycerol
• Short-term storage: In neutral buffer at 4 °C
• Long-term storage: In neutral buffer with 20% ethanol at 4 °C

Required equipment:
- Lysis buffer
- Wash buffer
- Elution buffer (incl. Rho1D4 peptide)
- Ice bath
- Refrigerated centrifuge for 50 ml tube (min 10000×g)
- 50 ml centrifuge tube
- Micropipettor and micropipetting tips
- Disposable gravity flow columns with capped bottom outlet, 2 ml
- pH meter
- End-over-end shaker
- SDS-PAGE buffers, reagents and equipment optional: Western Blot reagents and equipment

The Rho starter set 2 is meant for protein purification assays of the highest possible purity, exploiting the specificity of an antibody to its antigen.