The PureCube Zn-NTA Compact cartridge is an IMAC chromatography column prepacked with PureCube Zn-NTA resin. You can choose between two sizes, 1 and 5 ml bed volume.

• Usage: Specific binding and purification of 6× His-tagged proteins
• Binding of Zinc-binding proteins
• Specificity: Affinity to His-tagged proteins
• Affinity to Zinc-finger proteins
• Binding capacity: >50 mg/ml
• Bead Ligand: Zn-NTA
• Bead size: 40 μm
• Chelator stability: Stable in buffer containing 10 mM DTT and 1 mM EDTA
• Filling quantity: Delivered as a 50% suspension
• Required equipment and materials:
• FPLC hardware like e.g. ÄKTA
• Binding Buffer
• Elution Buffer

Please have a look at the given features table to ensure that the Cartridge/Column is compatible with your FPLC hardware. Common systems like ÄKTA TM are compatible without problem. If this is not the case we recommend buying the sole loose PureCube Zn-NTA resin and packing the columns yourself. Alternatively, we also offer our PureCube Zn-NTA products as magnetic beads, if you prefer this IMAC method.