Our PureCube HiCap StrepTactin® agarose XL resin beads consist of 6% cross-linked agarose coupled to the StrepTactin protein.

• Usage: Highly specific affinity purification of Strep-(WSHPQFEK)-tagged proteins
• Specificity: Affinity to Strep-tagged (WSHPQFEK) proteins
• Binding capacity: >5 mg/ml
• Bead Ligand: StrepTactin®
• Bead size: 400 μm
• Matrix: 6% cross-linked agarose
• Filling quantity: Delivered as a 50% suspension
• Chemical stability: 2% Tween 20, 2% Triton X-100, 2% IGEPAL® 630/Nonidet P40, n-Octyl-beta-D-glucopyranoside, 0,2% n-Nonyl-beta-D-glucopyranoside, 0,35% N-Decyl-beta-D-maltoside, 2% Lauryl-sarcosine, 0, 1% SDS, 0,3% CHAPS, 1M Guanidine HCl, 1mM PMSF, 10% Ethanol, 5M NaCl, 2M (NH4)SO4, 1M CaCl2, 25% Glycerol
• Short-term storage: In neutral buffer at 4 °C
• Long-term storage: In neutral buffer with sodium azide at 4 °C
• Required equipment:
• Lysis Buffer
• Wash Buffer
• Elution Buffer (incl. Rho1D4 peptide)
• Ice bath
• Refrigerated centrifuge for 50 ml tube (min 10000×g)
• 50 ml centrifuge tube
• Micropipettor and Micropipetting tips
• Disposable gravity flow columns with capped bottom outlet, 2 ml
• pH meter
• End-over-end shaker
• SDS-PAGE buffers, reagents and equipment Optional: Western Blot reagents and equipment

The PureCube HiCap StrepTactin® agarose XL resin beads enable protein yields of up to 5 mg protein per ml resin and were developed for FPLC and batch spin-based protein purification.