PureCube Maleimide Agarose resin is your best option to couple biomolecules that carry thiol (SH) groups.

• Usage: Coupling proteins to active Maleimide Groups
• Specificity: Exposed thiol (SH) groups on biomolecule
• Bead Ligand: Chemically active Maleimide groups
• Resulting Bond: irreversible Thioether
• Protein binding capacity: ≥15 mg/ml
• Bead size: 40 μm
• Filling quantity: Delivered as a 50% suspension
• Matrix: 7.5% cross-linked agarose
• Short Term Storage: In neutral buffer at 4 °C
• Long Term Storage: 100 mM sodium hydrogen carbonate, 0.02% sodium azide, pH 7.5 at 4 °C or 20 mM sodium acetate, 20% ethanol, pH 6.5 at 4 °C
• Shelf life: If properly stored - 3 months upon delivery, unopened
• Chemical Structure: Maleimide structure

The resulting Thioether bond between the Maleimide Agarose beads and your biomolecule of interest is stable so that the bead can be used for other applications afterward. Our PureCube Maleimide Activated Agarose is also available as magnetic beads if you prefer this method or as XL agarose resin if you need higher flow rates for your downstream application.