The protein purification with the PureCube Glutathione MagBeads and the GST-tag exploit the specific enzyme-substrate binding to gain high levels of purity while maintaining a high protein yield.

• Usage: Specific binding and purification of GST-tagged proteins
• Specificity: Affinity to GST(Glutathione-S-Transferase)-tagged proteins
• Binding capacity: >20 mg/ml
• Bead Ligand: L-Glutathione (reduced)
• Bead size: 30 μm
• Matrix: 6% cross-linked agarose
• Filling quantity: Delivered as a 25% suspension
• pH Stability: 3 to 12
• Stabilities: 1 M sodium acetate, pH 4.0, or 6 M guanidinehydrochloride, organic solvents (e.g., 70% (v/v) ethanol), 1% (w/v) SDS, 0.1 M NaOH, 0.1 M HCl

The pairing of an enzyme to its substrate is one of the most specific combinations in life sciences. PureCube Glutathione MagBeads is a suspension of tiny 30 µm magnetic purification beads that are used for this purpose. Their small size provides high mechanical stability. Additionally, we offer GST-tag affinity MagBeads with a bead diameter of 90 µm for very viscous cell media. Alternatively, Glutathione Agarose resins for FPLC or batch spin approaches are also available.