About this item
Authenticase™ is a proprietary mixture of structure-specific nucleases capable of recognizing and cleaving outside mismatch and indel (insertion and/or deletion) regions, ranging from 1 to 10 basepairs (bp) on double-stranded DNA.
- Cleave heteroduplex DNA
- Improve accuracy in gene synthesis
- Supplied with 5X DNA anneal buffer and 10X reaction buffer
Authenticase is a mixture of enzymes capable of recognizing mismatch and indel regions on a double-stranded DNA and creating double-stranded cleavages around these regions and with a limited non specific activity on homoduplex regions of DNA. The regions of mismatch or indel could range from at least 1 to 10 bps. The activity of cleavage on mismatch/Indel regions can be utilized in the process of oligo-based PCR gene synthesis to improve the accuracy of PCR amplicons. Alternatively, Authenticase™ treated products can be analyzed to determine the popularity of heteroduplex of a PCR pool amplified from enzyme-based edited genome.
To order chemicals, medical devices, or other restricted products please provide ID that includes your business name & shipping address via email [email protected] or fax 484.881.5997 referencing your VWR account number. Acceptable forms of ID are:
- • State issued document with your organization's Federal Tax ID Number
- • State issued document with your organization's Resale Tax ID Number
- • City or County issued Business License
- • State Department of Health Services License
- • Any other ID issued by the State that includes the business name & address
* ATTN: California Customers may require additional documentation as part of the CA Health & Safety Code. Products that fall under this regulation will be placed on a mandatory 21-day hold after documentation is received. Avantor will not lift restrictions for residential shipping addresses.