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Specifications
- Assay duration:Multiple steps
- Assay Type (ELISA with LOV):Indirect
- Format:Pre-coated
- Host:
- Primary antibody reactivity:HumanRatMouse
- Target protein:60S Ribosomal Protein L10
- Description:60S Ribosomal protein L10 cell based ELISA kit
- Size:96 tests
- Environmentally Preferable:
- Sample Type:Adherent cells and suspension cells
- Detection Method:Colorimetric
- Time to Results:4 h 30 min
- Shelf Life:Store for 6 months at 4 °C
- Detection Range:>5000 cells
- Storage Temperature:4 °C
- Regulatory Status:RUO
- Cat. No.:77211-986
Specifications
About this item
This 60S Ribosomal Protein L10 Cell Based ELISA kit allows for the detection of 60S Ribosomal Protein L10 and the effects that certain stimulation conditions have on 60S Ribosomal Protein L10 expression in different cell lines. Qualitative determination of 60S Ribosomal Protein L10 concentration is achieved by an indirect ELISA format. In essence, the 60S Ribosomal Protein L10 is captured by Anti-60S Ribosomal Protein L10 Antibodies which in turn are detected by HRP-conjugated secondary antibodies. Through this binding, the HRP enzyme (conjugated to the secondary antibody) can catalyze a colorimetric reaction upon substrate addition. Due to the qualitative nature of this 60S Ribosomal Protein L10 Cell Based ELISA kit, multiple normalization methods are described: 1) Anti-GAPDH Antibody is included to serve as an internal positive control in normalizing the target absorbance values. 2) Following the colorimetric measurement of HRP activity via substrate addition, the Crystal Violet whole-cell staining method is used to determine cell density. After staining, the results can be analyzed by normalizing the absorbance values to cell amounts, by which the plating difference can be adjusted.