Mouse Rab1A ELISA kit is a 90 minutes sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of mouse Rab1A in serum, plasma, and other biological fluids.

• Higher throughput: Get results in just 90 minutes, with a single wash step
• Detection range: 100 to 1600 ng/L
• Sensitivity: 7.23 ng/L
• Sample type: Serum, plasma or other biological fluids
• Assay precision: Intra-assay: CV <8%, Inter-assay: CV <10%

Mouse Rab1A ELISA kit (A314254) employs the sandwich enzyme immunoassay technique for the quantitative measurement of mouse Rab1A in serum, plasma or other biological fluids. An antibody specific for Rab1A has been pre-coated onto a 96-well microtiter plate. The standards and test samples are added into the wells and the Rab1A present in each sample is bound to the wells by the immobilized antibody. Biotinylated Anti-Rab1A antibody, which also binds the Rab1A present in each sample, and Streptavidin-HRP, which binds the Biotinylated Anti-Rab1A antibody, are added and the microtiter plate is incubated. Following incubation, unbound Biotinylated Anti-Rab1A antibody and unbound Streptavidin-HRP are removed by washing, and two substrate solutions are added to the wells. Color develops in proportion to the amount of Rab1A captured in each well. The color development is stopped by addition of stop solution which changes the color from blue to yellow and the intensity of the color is then measured. The concentration of Rab1A in the samples can then be calculated by reading the O.D. absorbance at 450 nm in a microplate reader and referring to the standard curve.