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Human Methionyl Aminopeptidase 1/MAP 1 ELISA Kit
Catalog # 77203-456
Supplier:  ANTIBODIES.COM LLC
CAS Number:  
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Human Methionyl Aminopeptidase 1/MAP 1 ELISA Kit
Catalog # 77203-456
Supplier:  ANTIBODIES.COM LLC
Supplier Number:  A311286-96
CAS Number:  

Specifications

  • Assay duration:
    One step
  • Assay Type (ELISA with LOV):
    Sandwich
  • Format:
    Pre-coated
  • Primary antibody reactivity:
    Human
  • Target protein:
    Methionyl Aminopeptidase 1/MAP 1
  • Description:
    Human Methionyl Aminopeptidase 1/MAP 1 ELISA kit
  • Size:
    96 tests
  • Sample Type:
    Serum, plasma or other biological fluids
  • Detection Method:
    Colorimetric
  • Time to Results:
    1 h 30 min
  • Shelf Life:
    Store for 6 months at 4 °C
  • Detection Range:
    0.5 - 8 ng/ml
  • Storage Temperature:
    4 °C
  • Sample Volume:
    40 μl
  • Sensitivity:
    0.017 ng/ml
  • Regulatory Status:
    RUO
  • Cat. No.:
    77203-456

Specifications

About this item

Human Methionyl Aminopeptidase 1/MAP 1 ELISA kit is a 90 minutes sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of human Methionyl Aminopeptidase 1/MAP 1 in serum, plasma, and other biological fluids.

  • Higher throughput: Get results in just 90 minutes, with a single wash step
  • Detection range: 0.5 to 8 ng/ml
  • Sensitivity: 0.017 ng/ml
  • Sample type: Serum, plasma or other biological fluids
  • Assay precision: Intra-assay: CV <8%, Inter-assay: CV <10%

Human Methionyl Aminopeptidase 1/MAP 1 ELISA kit (A311286) employs the sandwich enzyme immunoassay technique for the quantitative measurement of human Methionyl Aminopeptidase 1/MAP 1 in serum, plasma or other biological fluids. An antibody specific for Methionyl Aminopeptidase 1/MAP 1 has been pre-coated onto a 96-well microtiter plate. The standards and test samples are added into the wells and the Methionyl Aminopeptidase 1/MAP 1 present in each sample is bound to the wells by the immobilized antibody. Biotinylated Anti-Methionyl Aminopeptidase 1/MAP 1 antibody, which also binds the Methionyl Aminopeptidase 1/MAP 1 present in each sample, and Streptavidin-HRP, which binds the Biotinylated Anti-Methionyl Aminopeptidase 1/MAP 1 antibody, are added and the microtiter plate is incubated. Following incubation, unbound Biotinylated Anti-Methionyl Aminopeptidase 1/MAP 1 antibody and unbound Streptavidin-HRP are removed by washing, and two substrate solutions are added to the wells. Color develops in proportion to the amount of Methionyl Aminopeptidase 1/MAP 1 captured in each well. The color development is stopped by addition of stop solution which changes the color from blue to yellow and the intensity of the color is then measured. The concentration of Methionyl Aminopeptidase 1/MAP 1 in the samples can then be calculated by reading the O.D. absorbance at 450 nm in a microplate reader and referring to the standard curve.

Ordering information: This is a ready-to-use ELISA kit that contains: Pre-coated 96 well microplate, standard solution, standard diluent, biotinylated detection antibody, streptavidin-HRP, wash buffer (25X), substrate solution A, substrate solution B, stop solution, plate sealers, foil pouch, and protocol manual.