Human KIF22 ELISA kit is a 90 minutes sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of human KIF22 in serum, plasma, and other biological fluids.

• Higher throughput: Get results in just 90 minutess, with a single wash step
• Detection Range: 125 to 2000 ng/L
• Sensitivity: 11.19 ng/L
• Sample Type: Serum, plasma or other biological fluids
• Assay precision: Intra assay: CV <8%, Inter assay: CV <10%

Human KIF22 ELISA kit employs the sandwich enzyme immunoassay technique for the quantitative measurement of human KIF22 in serum, plasma or other biological fluids. An antibody specific for KIF22 has been pre-coated onto a 96-well microtiter plate. The standards and test samples are added into the wells and the KIF22 present in each sample is bound to the wells by the immobilized antibody. Biotinylated anti-KIF22 antibody, which also binds the KIF22 present in each sample, and Streptavidin-HRP, which binds the Biotinylated anti-KIF22 antibody, are added and the microtiter plate is incubated. Following incubation, unbound Biotinylated anti-KIF22 antibody and unbound Streptavidin-HRP are removed by washing, and two substrate solutions are added to the wells. Color develops in proportion to the amount of KIF22 captured in each well. The color development is stopped by addition of stop solution which changes the color from blue to yellow and the intensity of the color is then measured. The concentration of KIF22 in the samples can then be calculated by reading the O.D. absorbance at 450 nm in a microplate reader and referring to the standard curve.