Mouse IL-13 Receptor alpha 1 ELISA kit is a 90 minutes sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of mouse IL-13 Receptor alpha 1 in serum, plasma, and other biological fluids.

• Higher throughput: Get results in just 90 minutess, with a single wash step
• Detection Range: 150to2400 ng/L
• Sensitivity: 8.99 ng/L
• Sample Type: Serum, plasma or other biological fluids
• Assay Precision: Intra-assay: CV <8%, Inter-assay: CV <10%

Mouse IL-13 Receptor alpha 1 ELISA kit (A310631) employs the sandwich enzyme immunoassay technique for the quantitative measurement of mouse IL-13 Receptor alpha 1 in serum, plasma or other biological fluids. An antibody specific for IL-13 Receptor alpha 1 has been pre-coated onto a 96-well microtiter plate. The standards and test samples are added into the wells and the IL-13 Receptor alpha 1 present in each sample is bound to the wells by the immobilized antibody. Biotinylated anti-IL-13 Receptor alpha 1 antibody, which also binds the IL-13 Receptor alpha 1 present in each sample, and Streptavidin-HRP, which binds the Biotinylated anti-IL-13 Receptor alpha 1 antibody, are added and the microtiter plate is incubated. Following incubation, unbound Biotinylated anti-IL-13 Receptor alpha 1 antibody and unbound Streptavidin-HRP are removed by washing, and two substrate solutions are added to the wells. Color develops in proportion to the amount of IL-13 Receptor alpha 1 captured in each well. The color development is stopped by addition of stop solution which changes the color from blue to yellow and the intensity of the color is then measured. The concentration of IL-13 Receptor alpha 1 in the samples can then be calculated by reading the O.D. absorbance at 450 nm in a microplate reader and referring to the standard curve.