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Human EMAP II/AIMP1 ELISA Kit
  77213-842
 :  
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Human EMAP II/AIMP1 ELISA Kit
  77213-842
 :  A310321-96
 :  
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To order chemicals, medical devices, or other restricted products please provide ID that includes your business name & shipping address via email [email protected] or fax 484.881.5997 referencing your VWR account number. Acceptable forms of ID are:

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  • • Any other ID issued by the State that includes the business name & address

* ATTN: California Customers may require additional documentation as part of the CA Health & Safety Code. Products that fall under this regulation will be placed on a mandatory 21-day hold after documentation is received. Avantor will not lift restrictions for residential shipping addresses.

 

  • Sample Type:
    Serum, plasma or other biological fluids
  • Detection Method:
    Colorimetric
  • Time to Results:
    1 h 30 min
  • Shelf Life:
    Store for 6 months at 4 °C
  • Detection Range:
    100 - 1600 ng/L
  • Storage Temperature:
    4 °C
  • Sample Volume:
    40 μl
  • Sensitivity:
    7.39 ng/L
  • Regulatory Status:
    RUO
  • Cat. No.:
    77213-842

 

 

Human EMAP II/AIMP1 ELISA kit is a 90 minutes sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of human EMAP II/AIMP1 in serum, plasma, and other biological fluids.

  • Higher throughput: Get results in just 90 minutess, with a single wash step
  • Detection Range: 100to1600 ng/L
  • Sensitivity: 7.39 ng/L
  • Sample Type: Serum, plasma or other biological fluids
  • Assay Precision: Intra-assay: CV <8%, Inter-assay: CV <10%

Human EMAP II/AIMP1 ELISA kit (A310321) employs the sandwich enzyme immunoassay technique for the quantitative measurement of human EMAP II/AIMP1 in serum, plasma or other biological fluids. An antibody specific for EMAP II/AIMP1 has been pre-coated onto a 96-well microtiter plate. The standards and test samples are added into the wells and the EMAP II/AIMP1 present in each sample is bound to the wells by the immobilized antibody. Biotinylated anti-EMAP II/AIMP1 antibody, which also binds the EMAP II/AIMP1 present in each sample, and Streptavidin-HRP, which binds the Biotinylated anti-EMAP II/AIMP1 antibody, are added and the microtiter plate is incubated. Following incubation, unbound Biotinylated anti-EMAP II/AIMP1 antibody and unbound Streptavidin-HRP are removed by washing, and two substrate solutions are added to the wells. Color develops in proportion to the amount of EMAP II/AIMP1 captured in each well. The color development is stopped by addition of stop solution which changes the color from blue to yellow and the intensity of the color is then measured. The concentration of EMAP II/AIMP1 in the samples can then be calculated by reading the O.D. absorbance at 450 nm in a microplate reader and referring to the standard curve.

 : This is a ready-to-use ELISA kit that contains: Pre-coated 96 well microplate, standard solution, standard diluent, biotinylated detection antibody, streptavidin-HRP, wash buffer (25X), substrate solution A, substrate solution B, stop solution, plate sealers, foil pouch, and protocol manual.