Human Cytochrome C Oxidase Subunit VIc/COX6C ELISA kit is a 90 minutes sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of human Cytochrome C Oxidase Subunit VIc/COX6C in serum, plasma, and other biological fluids.

• Higher throughput: Get results in just 90 minutess, with a single wash step
• Detection Range: 250 - 4000 ng/L
• Sensitivity: 13.68 ng/L
• Sample Type: Serum, plasma or other biological fluids
• Assay Precision: Intra - Assay: CV <8%, Inter - Assay: CV <10%

Human Cytochrome C Oxidase Subunit VIc/COX6C ELISA kit (A313591) employs the sandwich enzyme immunoassay technique for the quantitative measurement of human Cytochrome C Oxidase Subunit VIc/COX6C in serum, plasma or other biological fluids. An antibody specific for Cytochrome C Oxidase Subunit VIc/COX6C has been pre-coated onto a 96-well microtiter plate. The standards and test samples are added into the wells and the Cytochrome C Oxidase Subunit VIc/COX6C present in each sample is bound to the wells by the immobilized antibody. Biotinylated anti-Cytochrome C Oxidase Subunit VIc/COX6C antibody, which also binds the Cytochrome C Oxidase Subunit VIc/COX6C present in each sample, and Streptavidin-HRP, which binds the Biotinylated anti-Cytochrome C Oxidase Subunit VIc/COX6C antibody, are added and the microtiter plate is incubated. Following incubation, unbound Biotinylated anti-Cytochrome C Oxidase Subunit VIc/COX6C antibody and unbound Streptavidin-HRP are removed by washing, and two substrate solutions are added to the wells. Color develops in proportion to the amount of Cytochrome C Oxidase Subunit VIc/COX6C captured in each well. The color development is stopped by addition of stop solution which changes the color from blue to yellow and the intensity of the color is then measured. The concentration of Cytochrome C Oxidase Subunit VIc/COX6C in the samples can then be calculated by reading the O.D. absorbance at 450 nm in a microplate reader and referring to the standard curve.