Human ATP5A ELISA kit is a 90 minute sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of human ATP5A in serum, plasma, and other biological fluids.

• Higher throughput: Get results in just 90 minutes, with a single wash step
• Assay precision: Intra-assay: CV <8%, Inter-assay: CV <10%

Human ATP5A ELISA kit (A310691) employs the sandwich enzyme immunoassay technique for the quantitative measurement of human ATP5A in serum, plasma or other biological fluids. An antibody specific for ATP5A has been pre-coated onto a 96-well microtiter plate. The standards and test samples are added into the wells and the ATP5A present in each sample is bound to the wells by the immobilized antibody. Biotinylated anti-ATP5A antibody, which also binds the ATP5A present in each sample, and Streptavidin-HRP, which binds the Biotinylated anti-ATP5A antibody, are added and the microtiter plate is incubated. Following incubation, unbound Biotinylated anti-ATP5A antibody and unbound Streptavidin-HRP are removed by washing, and two substrate solutions are added to the wells. Color develops in proportion to the amount of ATP5A captured in each well. The color development is stopped by addition of stop solution which changes the color from blue to yellow and the intensity of the color is then measured. The concentration of ATP5A in the samples can then be calculated by reading the O.D. absorbance at 450 nm in a microplate reader and referring to the standard curve.