- Assay duration:One step
- Assay Type (ELISA with LOV):Sandwich
- Format:Pre-coated
- Primary antibody reactivity:Human
- Target protein:Androgen Receptor
- Description:Human androgen receptor ELISA kit
- Size:96 tests
- Sample Type:Serum, plasma or other biological fluids
- Detection Method:Colorimetric
- Time to Results:1 h 30 min
- Shelf Life:Store for 6 months at 4 °C
- Detection Range:2.5 - 40 ng/ml
- Storage Temperature:4 °C
- Sample Volume:40 μl
- Sensitivity:0.095 ng/ml
- Regulatory Status:RUO
- Cat. No.:77209-494
Human Androgen receptor ELISA kit is a 90 minute sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of human Androgen receptor in serum, plasma, and other biological fluids.
- Higher throughput: Get results in just 90 minutes, with a single wash step
- Assay precision: Intra-assay: CV <8%, Inter-assay: CV <10%
Human Androgen receptor ELISA kit (A310812) employs the sandwich enzyme immunoassay technique for the quantitative measurement of human Androgen receptor in serum, plasma or other biological fluids. An antibody specific for Androgen receptor has been pre-coated onto a 96-well microtiter plate. The standards and test samples are added into the wells and the Androgen receptor present in each sample is bound to the wells by the immobilized antibody. Biotinylated anti-Androgen receptor antibody, which also binds the Androgen receptor present in each sample, and Streptavidin-HRP, which binds the Biotinylated anti-Androgen receptor antibody, are added and the microtiter plate is incubated. Following incubation, unbound Biotinylated anti-Androgen receptor antibody and unbound Streptavidin-HRP are removed by washing, and two substrate solutions are added to the wells. Color develops in proportion to the amount of Androgen receptor captured in each well. The color development is stopped by addition of stop solution which changes the color from blue to yellow and the intensity of the color is then measured. The concentration of Androgen receptor in the samples can then be calculated by reading the O.D. absorbance at 450 nm in a microplate reader and referring to the standard curve.