- Assay duration:Multiple steps
- Assay Type (ELISA with LOV):Sandwich
- Format:Pre-coated
- Primary antibody reactivity:Other
- Target protein:Resistin
- Description:Sheep resistin ELISA kit
- Size:96 tests
- Sample Type:Serum, plasma, tissue homogenates and other biological fluids
- Cross Reactivity:Sheep Resistin ELISA Kit exhibits high specificity and excellent specificity for the detection of sheep Resistin. No significant cross-reactivity or interference between Resistin and analogues was observed.
- Detection Method:Colorimetric
- Time to Results:4 h 30 min
- Assay Principle:Quantitative
- Shelf Life:Store for 6 months at 4 °C
- Detection Range:0.469 - 30 ng/ml
- Storage Temperature:4 °C
- Sample Volume:100 μl
- Sensitivity:0.281 ng/ml
- Regulatory Status:RUO
- Cat. No.:76747-246
Sheep Resistin ELISA kit is a sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of sheep Resistin in serum, plasma, tissue homogenates, and other biological fluids.
- Ready To Use ELISA kit
- Detection Range: 0.469 to 30 ng/ml
- Sensitivity: 0.281 ng/ml
- Sample Type: Serum, plasma, tissue homogenates and other biological fluids
- Assay Precision: Intra - Assay: CV <8%, Inter - Assay: CV <10%
Sheep Resistin ELISA kit (A77609) employs the sandwich enzyme immunoassay technique for the quantitative measurement of sheep Resistin in serum, plasma, tissue homogenates, and other biological fluids. An antibody specific for Resistin has been pre-coated onto a 96-well microtiter plate. The standards and test samples are added into the wells and the Resistin present in each sample is bound to the wells by the immobilized antibody. Following incubation, the wells are washed and then incubated with Biotinylated Anti-Resistin Antibody, which binds the captured Resistin present in each well. Following incubation, unbound biotinylated detection antibody is removed by washing, and an HRP-Streptavidin conjugate is added to the wells and the microtiter plate is incubated. Following incubation and washing, TMB substrate solution is then used to visualize the HRP enzymatic reaction by catalysis to produce a blue-coloured product that changes to yellow after addition of acidic stop solution. The density of yellow is proportional to the amount of Resistin captured in each well. The concentration of Resistin can then be calculated by reading the O.D. absorbance at 450 nm in a microplate reader and referring to the standard curve.