Rat Rab11A ELISA kit is a competitive Enzyme-Linked Immunosorbent Assay (cELISA) designed for the in vitro quantitative determination of rat Rab11A in serum, plasma, tissue homogenates, and other biological fluids.

• Ready to use ELISA kit
• Detection range: 1.563 to 100 ng/ml
• Sensitivity: 0.938 ng/ml
• Sample type: Serum, plasma, tissue homogenates and other biological fluids
• Assay precision: Intra assay: CV <8%, Inter assay: CV <10%

Rat Rab11A ELISA kit (A78706) employs the competitive enzyme immunoassay technique for the quantitative measurement of rat Rab11A in serum, plasma, tissue homogenates, and other biological fluids. The 96-well microtiter plate has been pre-coated with Rab11A antigen. During the incubation, Rab11A present in the samples or standards competes with the fixed amount of immobilized Rab11A for binding sites on the Biotinylated Anti-Rab11A Antibody. The more Rab11A present in a sample or standard, the less Biotinylated Anti-Rab11A Antibody that binds to the plate. Following incubation, unbound Biotinylated Anti-Rab11A Antibody is removed by washing, and an HRP-Streptavidin conjugate is added to the wells and the microtiter plate is incubated. Following incubation and washing, TMB substrate solution is then used to visualize the HRP enzymatic reaction by catalysis to produce a blue-coloured product that changes to yellow after addition of acidic stop solution. The density of yellow is inversely proportional to the amount of Rab11A present in each sample or standard. The concentration of Rab11A can then be calculated by reading the O.D. absorbance at 450 nm in a microplate reader and referring to the standard curve.