- Assay duration:Multiple steps
- Assay Type (ELISA with LOV):Sandwich
- Format:Pre-coated
- Primary antibody reactivity:Monkey
- Target protein:CCL11/Eotaxin
- Description:Monkey CCL11/Eotaxin ELISA kit
- Size:96 tests
- Sample Type:Serum, plasma, tissue homogenates and other biological fluids
- Cross Reactivity:Monkey CCL11/Eotaxin ELISA kit exhibits high specificity and excellent specificity for the detection of monkey CCL11/Eotaxin. No significant cross-reactivity or interference between CCL11/Eotaxin and analogues was observed.
- Detection Method:Colorimetric
- Time to Results:4 h 30 min
- Assay Principle:Quantitative
- Shelf Life:Store for 6 months at 4 °C
- Detection Range:15.625 - 1000 pg/ml
- Storage Temperature:4 °C
- Sample Volume:100 μl
- Sensitivity:9.375 pg/ml
- Regulatory Status:RUO
- Cat. No.:76744-236
Monkey CCL11/Eotaxin ELISA kit is a sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of monkey CCL11/Eotaxin in serum, plasma, tissue homogenates, and other biological fluids.
- Ready-To-Use ELISA Kit
- Detection Range: 15.625 - 1000 pg/ml
- Sensitivity: 9.375 pg/ml
- Sample Type: Serum, plasma, tissue homogenates, and other biological fluids
- Assay Precision: Intra-Assay: CV <8%, Inter-Assay: CV <10%
Monkey CCL11/Eotaxin ELISA kit (A73899) employs the sandwich enzyme immunoassay technique for the quantitative measurement of monkey CCL11/Eotaxin in serum, plasma, tissue homogenates, and other biological fluids. An antibody specific for CCL11/Eotaxin has been pre-coated onto a 96-well microtiter plate. The standards and test samples are added into the wells and the CCL11/Eotaxin present in each sample is bound to the wells by the immobilized antibody. Following incubation, the wells are washed and then incubated with Biotinylated Anti-CCL11/Eotaxin Antibody, which binds the captured CCL11/Eotaxin present in each well. Following incubation, unbound biotinylated detection antibody is removed by washing, and an HRP-Streptavidin conjugate is added to the wells and the microtiter plate is incubated. Following incubation and washing, TMB substrate solution is then used to visualize the HRP enzymatic reaction by catalysis to produce a blue-coloured product that changes to yellow after addition of acidic stop solution. The density of yellow is proportional to the amount of CCL11/Eotaxin captured in each well. The concentration of CCL11/Eotaxin can then be calculated by reading the O.D. absorbance at 450 nm in a microplate reader and referring to the standard curve.