Specifications
- Assay duration:Multiple steps
- Assay Type (ELISA with LOV):Sandwich
- Format:Pre-coated
- Primary antibody reactivity:Mouse
- Target protein:Apolipoprotein B100
- Description:Mouse apolipoprotein B100 ELISA kit
- Size:96 tests
- Sample Type:Serum, plasma, tissue homogenates and other biological fluids
- Cross Reactivity:Mouse Apolipoprotein B100 ELISA kit exhibits high specificity and excellent specificity for the detection of mouse Apolipoprotein B100. No significant cross-reactivity or interference between Apolipoprotein B100 and analogues was observed.
- Detection Method:Colorimetric
- Time to Results:4 h 30 min
- Assay Principle:Quantitative
- Shelf Life:Store for 6 months at 4 °C
- Detection Range:78.125 - 5000 ng/ml
- Storage Temperature:4 °C
- Sample Volume:100 μl
- Sensitivity:46.875 ng/ml
- Regulatory Status:RUO
- Cat. No.:76743-276
Specifications
About this item
Mouse Apolipoprotein B100 ELISA kit is a sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of mouse Apolipoprotein B100 in serum, plasma, tissue homogenates, and other biological fluids.
- Ready-To-Use ELISA Kit
- Detection Range: 78.125 - 5000 ng/ml
- Sensitivity: 46.875 ng/ml
- Sample Type: Serum, plasma, tissue homogenates, and other biological fluids
- Assay Precision: Intra-Assay: CV <8%, Inter-Assay: CV <10%
Mouse Apolipoprotein B100 ELISA kit (A74338) employs the sandwich enzyme immunoassay technique for the quantitative measurement of mouse Apolipoprotein B100 in serum, plasma, tissue homogenates, and other biological fluids. An antibody specific for Apolipoprotein B100 has been pre-coated onto a 96-well microtiter plate. The standards and test samples are added into the wells and the Apolipoprotein B100 present in each sample is bound to the wells by the immobilized antibody. Following incubation, the wells are washed and then incubated with Biotinylated Anti-Apolipoprotein B100 Antibody, which binds the captured Apolipoprotein B100 present in each well. Following incubation, unbound biotinylated detection antibody is removed by washing, and an HRP-Streptavidin conjugate is added to the wells and the microtiter plate is incubated. Following incubation and washing, TMB substrate solution is then used to visualize the HRP enzymatic reaction by catalysis to produce a blue-coloured product that changes to yellow after addition of acidic stop solution. The density of yellow is proportional to the amount of Apolipoprotein B100 captured in each well. The concentration of Apolipoprotein B100 can then be calculated by reading the O.D. absorbance at 450 nm in a microplate reader and referring to the standard curve.