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- Assay duration:Multiple steps
- Assay Type (ELISA with LOV):Sandwich
- Format:Pre-coated
- Host:
- Primary antibody reactivity:Rat
- Target protein:Surfactant Protein D/SP-D
- Description:Rat surfactant protein D/SP-D ELISA kit
- Size:96 tests
- Environmentally Preferable:
- Sample Type:Serum, plasma, tissue homogenates and other biological fluids
- Cross Reactivity:Rat Surfactant protein D/SP-D ELISA kit exhibits high specificity and excellent specificity for the detection of rat Surfactant Protein D/SP-D. No significant cross-reactivity or interference between Surfactant Protein D/SP-D and analogues was observed.
- Detection Method:Colorimetric
- Time to Results:4 h 30 min
- Assay Principle:Quantitative
- Shelf Life:Store for 6 months at 4 °C
- Detection Range:78.125 - 5000 pg/ml
- Storage Temperature:4 °C
- Sample Volume:100 μl
- Sensitivity:46.875 pg/ml
- Regulatory Status:RUO
- Cat. No.:76742-516
Rat Surfactant Protein D/SP-D ELISA kit is a sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of rat Surfactant Protein D/SP-D in serum, plasma, tissue homogenates, and other biological fluids.
- Ready-To-Use ELISA Kit
- Detection Range: 78.125 - 5000 pg/ml
- Sensitivity: 46.875 pg/ml
- Sample Type: Serum, plasma, tissue homogenates, and other biological fluids
- Assay Precision: Intra-Assay: CV <8%, Inter-Assay: CV <10%
Rat Surfactant protein D/SP-D ELISA kit (A80168) employs the sandwich enzyme immunoassay technique for the quantitative measurement of rat Surfactant Protein D/SP-D in serum, plasma, tissue homogenates, and other biological fluids. An antibody specific for Surfactant Protein D/SP-D has been pre-coated onto a 96-well microtiter plate. The standards and test samples are added into the wells and the Surfactant Protein D/SP-D present in each sample is bound to the wells by the immobilized antibody. Following incubation, the wells are washed and then incubated with Biotinylated Anti-Surfactant Protein D/SP-D Antibody, which binds the captured Surfactant Protein D/SP-D present in each well. Following incubation, unbound biotinylated detection antibody is removed by washing, and an HRP-Streptavidin conjugate is added to the wells and the microtiter plate is incubated. Following incubation and washing, TMB substrate solution is then used to visualize the HRP enzymatic reaction by catalysis to produce a blue-coloured product that changes to yellow after addition of acidic stop solution. The density of yellow is proportional to the amount of Surfactant Protein D/SP-D captured in each well. The concentration of Surfactant Protein D/SP-D can then be calculated by reading the O.D. absorbance at 450 nm in a microplate reader and referring to the standard curve.