Specifications
- Assay duration:Multiple steps
- Assay Type (ELISA with LOV):Sandwich
- Format:Pre-coated
- Primary antibody reactivity:Mouse
- Target protein:NCOR2/SMRT
- Description:Mouse NCOR2/SMRT ELISA kit
- Size:96 tests
- Sample Type:Serum, plasma, tissue homogenates and other biological fluids
- Cross Reactivity:Mouse NCOR2/SMRT ELISA Kit exhibits high specificity and excellent specificity for the detection of mouse NCOR2/SMRT. No significant cross-reactivity or interference between NCOR2/SMRT and analogues was observed.
- Detection Method:Colorimetric
- Time to Results:4 h 30 min
- Assay Principle:Quantitative
- Shelf Life:Store for 6 months at 4 °C
- Detection Range:62.5 - 4000 pg/ml
- Storage Temperature:4 °C
- Sample Volume:100 μl
- Sensitivity:37.5 pg/ml
- Regulatory Status:RUO
- Cat. No.:76739-606
Specifications
About this item
Mouse NCOR2/SMRT ELISA kit is a sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of mouse NCOR2/SMRT in serum, plasma, tissue homogenates, and other biological fluids.
- Ready To Use ELISA kit
- Detection Range: 62.5 to 4000 pg/ml
- Sensitivity: 37.5 pg/ml
- Sample Type: Serum, plasma, tissue homogenates and other biological fluids
- Assay Precision: Intra - Assay: CV <8%, Inter - Assay: CV <10%
Mouse NCOR2/SMRT ELISA kit (A77022) employs the sandwich enzyme immunoassay technique for the quantitative measurement of mouse NCOR2/SMRT in serum, plasma, tissue homogenates, and other biological fluids. An antibody specific for NCOR2/SMRT has been pre-coated onto a 96-well microtiter plate. The standards and test samples are added into the wells and the NCOR2/SMRT present in each sample is bound to the wells by the immobilized antibody. Following incubation, the wells are washed and then incubated with Biotinylated Anti-NCOR2/SMRT Antibody, which binds the captured NCOR2/SMRT present in each well. Following incubation, unbound biotinylated detection antibody is removed by washing, and an HRP-Streptavidin conjugate is added to the wells and the microtiter plate is incubated. Following incubation and washing, TMB substrate solution is then used to visualize the HRP enzymatic reaction by catalysis to produce a blue-coloured product that changes to yellow after addition of acidic stop solution. The density of yellow is proportional to the amount of NCOR2/SMRT captured in each well. The concentration of NCOR2/SMRT can then be calculated by reading the O.D. absorbance at 450 nm in a microplate reader and referring to the standard curve.