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Sheep Complement C3a ELISA Kit
Sheep Complement C3a ELISA Kit
  76735-596
 :  ANTIBODIES.COM LLC
 :  
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Sheep Complement C3a ELISA Kit
  76735-596
 :  ANTIBODIES.COM LLC
 :  A76223-96
 :  

 

  • Assay duration:
    Multiple steps
  • Assay Type (ELISA with LOV):
    Sandwich
  • Format:
    Pre-coated
  • Primary antibody reactivity:
    Other
  • Target protein:
    Complement C3a
  • Description:
    Sheep complement C3a ELISA kit
  • Size:
    96 tests
  • Sample Type:
    Serum, plasma, tissue homogenates and other biological fluids
  • Cross Reactivity:
    Sheep Complement C3a ELISA kit exhibits high specificity and excellent specificity for the detection of sheep Complement C3a. No significant cross-reactivity or interference between Complement C3a and analogues was observed.
  • Detection Method:
    Colorimetric
  • Time to Results:
    4 h 30 min
  • Assay Principle:
    Quantitative
  • Shelf Life:
    Store for 6 months at 4 °C
  • Detection Range:
    0.156 - 10 ng/ml
  • Storage Temperature:
    4 °C
  • Sample Volume:
    100 μl
  • Sensitivity:
    0.094 ng/ml
  • Regulatory Status:
    RUO
  • Cat. No.:
    76735-596

 

 

Sheep Complement C3a ELISA kit is a sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of sheep Complement C3a in serum, plasma, tissue homogenates, and other biological fluids.

  • Ready to use ELISA kit
  • Detection range: 0.156 to 10 ng/ml
  • Sensitivity: 0.094 ng/ml
  • Sample type: Serum. plasma. tissue homogenates. and other biological fluids
  • Assay precision: Intra assay: CV <8%. Inter assay: CV <10%

Sheep Complement C3a ELISA kit (A76223) employs the sandwich enzyme immunoassay technique for the quantitative measurement of sheep Complement C3a in serum, plasma, tissue homogenates, and other biological fluids. An antibody specific for Complement C3a has been pre-coated onto a 96-well microtiter plate. The standards and test samples are added into the wells and the Complement C3a present in each sample is bound to the wells by the immobilized antibody. Following incubation, the wells are washed and then incubated with Biotinylated Anti-Complement C3a Antibody, which binds the captured Complement C3a present in each well. Following incubation, unbound biotinylated detection antibody is removed by washing, and an HRP-Streptavidin conjugate is added to the wells and the microtiter plate is incubated. Following incubation and washing, TMB substrate solution is then used to visualize the HRP enzymatic reaction by catalysis to produce a blue-coloured product that changes to yellow after addition of acidic stop solution. The density of yellow is proportional to the amount of Complement C3a captured in each well. The concentration of Complement C3a can then be calculated by reading the O.D. absorbance at 450 nm in a microplate reader and referring to the standard curve.

 : This is a ready to use ELISA kit that contains: Pre-coated 96 well microplate, lyophilised standard, sample dilution buffer, biotinylated detection antibody (100X), antibody dilution buffer, HRP-Streptavidin conjugate (SABC) (100X), SABC dilution buffer, TMB substrate, stop solution, wash buffer (25X), plate sealers, foil pouch, and protocol manual.