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- Assay duration:Multiple steps
- Assay Type (ELISA with LOV):Sandwich
- Format:Pre-coated
- Primary antibody reactivity:Mouse
- Target protein:Methionine Aminopeptidase 2/p67
- Description:Mouse methionine aminopeptidase 2/p67 ELISA kit
- Size:96 tests
- Sample Type:Serum, plasma, tissue homogenates and other biological fluids
- Cross Reactivity:Mouse Methionine Aminopeptidase 2/p67 ELISA kit exhibits high specificity and excellent specificity for the detection of mouse Methionine Aminopeptidase 2/p67. No significant cross-reactivity or interference between Methionine Aminopeptidase 2/p67 and analogues was observed.
- Detection Method:Colorimetric
- Time to Results:4 h 30 min
- Assay Principle:Quantitative
- Shelf Life:Store for 6 months at 4 °C
- Detection Range:0.781 - 50 ng/ml
- Storage Temperature:4 °C
- Sample Volume:100 μl
- Sensitivity:0.469 ng/ml
- Regulatory Status:RUO
- Cat. No.:76731-216
Mouse Methionine Aminopeptidase 2/p67 ELISA kit is a competitive Enzyme-Linked Immunosorbent Assay (cELISA) designed for the in vitro quantitative determination of mouse Methionine Aminopeptidase 2/p67 in serum, plasma, tissue homogenates, and other biological fluids.
- Ready-To-Use ELISA Kit
- Detection Range: 0.781 - 50 ng/ml
- Sensitivity: 0.469 ng/ml
- Sample Type: Serum, plasma, tissue homogenates, and other biological fluids
- Assay Precision: Intra-Assay: CV <8%, Inter-Assay: CV <10%
Mouse Methionine Aminopeptidase 2/p67 ELISA kit (A75608) employs the sandwich enzyme immunoassay technique for the quantitative measurement of mouse Methionine Aminopeptidase 2/p67 in serum, plasma, tissue homogenates, and other biological fluids. An antibody specific for Methionine Aminopeptidase 2/p67 has been pre-coated onto a 96-well microtiter plate. The standards and test samples are added into the wells and the Methionine Aminopeptidase 2/p67 present in each sample is bound to the wells by the immobilized antibody. Following incubation, the wells are washed and then incubated with Biotinylated Anti-Methionine Aminopeptidase 2/p67 Antibody, which binds the captured Methionine Aminopeptidase 2/p67 present in each well. Following incubation, unbound biotinylated detection antibody is removed by washing, and an HRP-Streptavidin conjugate is added to the wells and the microtiter plate is incubated. Following incubation and washing, TMB substrate solution is then used to visualize the HRP enzymatic reaction by catalysis to produce a blue-coloured product that changes to yellow after addition of acidic stop solution. The density of yellow is proportional to the amount of Methionine Aminopeptidase 2/p67 captured in each well. The concentration of Methionine Aminopeptidase 2/p67 can then be calculated by reading the O.D. absorbance at 450 nm in a microplate reader and referring to the standard curve.