Specifications
- Assay duration:Multiple steps
- Assay Type (ELISA with LOV):Sandwich
- Format:Pre-coated
- Primary antibody reactivity:Dog
- Target protein:Interferon gamma
- Description:Canine interferon gamma ELISA kit
- Size:96 tests
- Sample Type:Serum, plasma, tissue homogenates and other biological fluids
- Cross Reactivity:Canine Interferon gamma ELISA kit exhibits high specificity and excellent specificity for the detection of canine Interferon gamma. No significant cross-reactivity or interference between Interferon gamma and analogues was observed.
- Detection Method:Colorimetric
- Time to Results:4 h 30 min
- Assay Principle:Quantitative
- Shelf Life:Store for 6 months at 4 °C
- Detection Range:62.5 - 4000 pg/ml
- Storage Temperature:4 °C
- Sample Volume:100 μl
- Sensitivity:37.5 pg/ml
- Regulatory Status:RUO
- Cat. No.:76744-642
Specifications
About this item
Canine Interferon gamma ELISA kit is a sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of canine Interferon gamma in serum, plasma, tissue homogenates, and other biological fluids.
- Ready-To-Use ELISA Kit
- Detection Range: 62.5 - 4000 pg/ml
- Sensitivity: 37.5 pg/ml
- Sample Type: Serum, plasma, tissue homogenates, and other biological fluids
- Assay Precision: Intra-Assay: CV <8%, Inter-Assay: CV <10%
Canine Interferon gamma ELISA kit (A74419) employs the sandwich enzyme immunoassay technique for the quantitative measurement of canine Interferon gamma in serum, plasma, tissue homogenates, and other biological fluids. An antibody specific for Interferon gamma has been pre-coated onto a 96-well microtiter plate. The standards and test samples are added into the wells and the Interferon gamma present in each sample is bound to the wells by the immobilized antibody. Following incubation, the wells are washed and then incubated with Biotinylated Anti-Interferon gamma Antibody, which binds the captured Interferon gamma present in each well. Following incubation, unbound biotinylated detection antibody is removed by washing, and an HRP-Streptavidin conjugate is added to the wells and the microtiter plate is incubated. Following incubation and washing, TMB substrate solution is then used to visualize the HRP enzymatic reaction by catalysis to produce a blue-coloured product that changes to yellow after addition of acidic stop solution. The density of yellow is proportional to the amount of Interferon gamma captured in each well. The concentration of Interferon gamma can then be calculated by reading the O.D. absorbance at 450 nm in a microplate reader and referring to the standard curve.