Specifications
- Assay duration:Multiple steps
- Assay Type (ELISA with LOV):Sandwich
- Format:Pre-coated
- Primary antibody reactivity:Bovine
- Target protein:Secretin/SCT
- Description:Bovine Secretin/SCT ELISA kit
- Size:96 tests
- Sample Type:Serum, plasma, tissue homogenates and other biological fluids
- Cross Reactivity:Bovine Secretin/SCT ELISA kit exhibits high specificity and excellent specificity for the detection of bovine Secretin/SCT. No significant cross-reactivity or interference between Secretin/SCT and analogues was observed.
- Detection Method:Colorimetric
- Time to Results:4 h 30 min
- Assay Principle:Quantitative
- Shelf Life:Store for 6 months at 4 °C
- Detection Range:7.813 - 500 pg/ml
- Storage Temperature:4 °C
- Sample Volume:100 μl
- Sensitivity:4.688 pg/ml
- Regulatory Status:RUO
- Cat. No.:76744-072
Specifications
About this item
Bovine Secretin/SCT ELISA kit is a sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of bovine Secretin/SCT in serum, plasma, tissue homogenates, and other biological fluids.
- Ready-To-Use ELISA Kit
- Detection Range: 7.813 - 500 pg/ml
- Sensitivity: 4.688 pg/ml
- Sample Type: Serum, plasma, tissue homogenates, and other biological fluids
- Assay Precision: Intra-Assay: CV <8%, Inter-Assay: CV <10%
Bovine Secretin/SCT ELISA kit (A87443) employs the sandwich enzyme immunoassay technique for the quantitative measurement of bovine Secretin/SCT in serum, plasma, tissue homogenates, and other biological fluids. An antibody specific for Secretin/SCT has been pre-coated onto a 96-well microtiter plate. The standards and test samples are added into the wells and the Secretin/SCT present in each sample is bound to the wells by the immobilized antibody. Following incubation, the wells are washed and then incubated with Biotinylated Anti-Secretin/SCT Antibody, which binds the captured Secretin/SCT present in each well. Following incubation, unbound biotinylated detection antibody is removed by washing, and an HRP-Streptavidin conjugate is added to the wells and the microtiter plate is incubated. Following incubation and washing, TMB substrate solution is then used to visualize the HRP enzymatic reaction by catalysis to produce a blue-coloured product that changes to yellow after addition of acidic stop solution. The density of yellow is proportional to the amount of Secretin/SCT captured in each well. The concentration of Secretin/SCT can then be calculated by reading the O.D. absorbance at 450 nm in a microplate reader and referring to the standard curve.