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6-keto-PGF1 alpha ELISA Kit
Catalog # 76743-672
Supplier:  ANTIBODIES.COM LLC
CAS Number:  
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6-keto-PGF1 alpha ELISA Kit
Catalog # 76743-672
Supplier:  ANTIBODIES.COM LLC
Supplier Number:  A73775-96
CAS Number:  

Specifications

  • Assay duration:
    Multiple steps
  • Assay Type (ELISA with LOV):
    Competitive
  • Format:
    Pre-coated
  • Primary antibody reactivity:
    Other
  • Target protein:
    6-keto-PGF1 alpha
  • Description:
    6-keto-PGF1 alpha ELISA kit
  • Size:
    96 tests
  • Sample Type:
    Serum, plasma, tissue homogenates and other biological fluids
  • Cross Reactivity:
    6-keto-PGF1 alpha ELISA Kit exhibits high specificity and excellent specificity for the detection of universal 6-keto-PGF1 alpha. No significant cross-reactivity or interference between 6-keto-PGF1 alpha and analogues was observed.
  • Detection Method:
    Colorimetric
  • Time to Results:
    4 h 30 min
  • Assay Principle:
    Quantitative
  • Shelf Life:
    Store for 6 months at 4 °C
  • Detection Range:
    15.625 - 1000 pg/ml
  • Storage Temperature:
    4 °C
  • Sample Volume:
    50 μl
  • Sensitivity:
    9.375 pg/ml
  • Regulatory Status:
    RUO
  • Cat. No.:
    76743-672

Specifications

About this item

6-keto-PGF1 alpha ELISA kit is a competitive Enzyme-Linked Immunosorbent Assay (cELISA) designed for the in vitro quantitative determination of 6-keto-PGF1 alpha in serum, plasma, tissue homogenates, and other biological fluids.

  • Ready To Use ELISA Kit
  • Detection Range: 15.625 to 1000 pg/ml
  • Sensitivity: 9.375 pg/ml
  • Sample Type: Serum, plasma, tissue homogenates, and other biological fluids
  • Assay Precision: Intra - Assay: CV <8%, Inter - Assay: CV <10%

6-keto-PGF1 alpha ELISA Kit (A73775) employs the competitive enzyme immunoassay technique for the quantitative measurement of universal 6-keto-PGF1 alpha in serum, plasma, tissue homogenates, and other biological fluids. The 96-well microtiter plate has been pre-coated with 6-keto-PGF1 alpha antigen. During the incubation, 6-keto-PGF1 alpha present in the samples or standards competes with the fixed amount of immobilized 6-keto-PGF1 alpha for binding sites on the Biotinylated Anti-6-keto-PGF1 alpha Antibody. The more 6-keto-PGF1 alpha present in a sample or standard, the less Biotinylated Anti-6-keto-PGF1 alpha Antibody that binds to the plate. Following incubation, unbound Biotinylated Anti-6-keto-PGF1 alpha Antibody is removed by washing, and an HRP-Streptavidin conjugate is added to the wells and the microtiter plate is incubated. Following incubation and washing, TMB substrate solution is then used to visualize the HRP enzymatic reaction by catalysis to produce a blue-coloured product that changes to yellow after addition of acidic stop solution. The density of yellow is inversely proportional to the amount of 6-keto-PGF1 alpha present in each sample or standard. The concentration of 6-keto-PGF1 alpha can then be calculated by reading the O.D. absorbance at 450nm in a microplate reader and referring to the standard curve.

Ordering information: This is a ready to use ELISA kit that contains: Pre-coated 96 well microplate, lyophilized standard, sample dilution buffer, biotinylated detection antibody (100X), antibody dilution buffer, HRP-Streptavidin conjugate (SABC) (100X), SABC dilution buffer, TMB substrate, stop solution, wash buffer (25X), plate sealers, foil pouch, and protocol manual.