Mouse gamma Secretase Subunit APH-1A ELISA kit is a sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of mouse gamma Secretase Subunit APH-1A in serum, plasma, tissue homogenates, and other biological fluids.

• Ready-To-Use ELISA Kit
• Detection Range: 1.563 - 100 ng/ml
• Sensitivity: 0.938 ng/ml
• Sample Type: Serum, plasma, tissue homogenates, and other biological fluids
• Assay Precision: Intra-Assay: CV <8%, Inter-Assay: CV <10%

Mouse gamma Secretase Subunit APH-1A ELISA kit (A76149) employs the sandwich enzyme immunoassay technique for the quantitative measurement of mouse gamma Secretase Subunit APH-1A in serum, plasma, tissue homogenates, and other biological fluids. An antibody specific for gamma Secretase Subunit APH-1A has been pre-coated onto a 96-well microtiter plate. The standards and test samples are added into the wells and the gamma Secretase Subunit APH-1A present in each sample is bound to the wells by the immobilized antibody. Following incubation, the wells are washed and then incubated with Biotinylated Anti-gamma Secretase Subunit APH-1A Antibody, which binds the captured gamma Secretase Subunit APH-1A present in each well. Following incubation, unbound biotinylated detection antibody is removed by washing, and an HRP-Streptavidin conjugate is added to the wells and the microtiter plate is incubated. Following incubation and washing, TMB substrate solution is then used to visualize the HRP enzymatic reaction by catalysis to produce a blue-coloured product that changes to yellow after addition of acidic stop solution. The density of yellow is proportional to the amount of gamma Secretase Subunit APH-1A captured in each well. The concentration of gamma Secretase Subunit APH-1A can then be calculated by reading the O.D. absorbance at 450 nm in a microplate reader and referring to the standard curve.