Human MASPIN ELISA Kit
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Product Details & Documents
Human MASPIN ELISA kit is a sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of human MASPIN in serum, plasma, tissue homogenates, and other biological fluids.
- Ready To Use ELISA Kit
- Detection Range: 1.563 to 100 ng/ml
- Sensitivity: 0.938 ng/ml
- Sample Type: Serum, plasma, tissue homogenates, and other biological fluids
- Assay Precision: Intra - Assay: CV <8%, Inter - Assay: CV <10%
Human MASPIN ELISA Kit (A74542) employs the sandwich enzyme immunoassay technique for the quantitative measurement of human MASPIN in serum, plasma, tissue homogenates, and other biological fluids. An antibody specific for MASPIN has been pre-coated onto a 96-well microtiter plate. The standards and test samples are added into the wells and the MASPIN present in each sample is bound to the wells by the immobilized antibody. Following incubation, the wells are washed and then incubated with Biotinylated Anti-MASPIN Antibody, which binds the captured MASPIN present in each well. Following incubation, unbound biotinylated detection antibody is removed by washing, and an HRP-Streptavidin conjugate is added to the wells and the microtiter plate is incubated. Following incubation and washing, TMB substrate solution is then used to visualize the HRP enzymatic reaction by catalysis to produce a blue-coloured product that changes to yellow after addition of acidic stop solution. The density of yellow is proportional to the amount of MASPIN captured in each well. The concentration of MASPIN can then be calculated by reading the O.D. absorbance at 450nm in a microplate reader and referring to the standard curve.
Ordering information: This is a ready to use ELISA kit that contains: Pre-coated 96 well microplate, lyophilized standard, sample dilution buffer, biotinylated detection antibody (100X), antibody dilution buffer, HRP-Streptavidin conjugate (SABC) (100X), SABC dilution buffer, TMB substrate, stop solution, wash buffer (25X), plate sealers, foil pouch, and protocol manual.
Documents
Specifications
Specifications
- Description:Human MASPIN ELISA kit
- Size:96 tests
- Primary antibody reactivity:Human
- Detection Range:1.563 - 100 ng/ml
- Sample Volume:100 μl
- Time to Results:4 h 30 min
- Detection Method:Colorimetric
- Assay Principle:Quantitative
- Cat. No.:76742-152
- Regulatory Status:RUO
- Assay duration:Multiple steps
- Sensitivity:0.938 ng/ml
- Format:Pre-coated
- Assay Type (ELISA with LOV):Sandwich
- Target protein:MASPIN
- Sample Type:Serum, plasma, tissue homogenates and other biological fluids
- Shelf Life:Store for 6 months at 4 °C
- Cross Reactivity:Human MASPIN ELISA Kit exhibits high specificity and excellent specificity for the detection of human MASPIN. No significant cross-reactivity or interference between MASPIN and analogues was observed.
- Storage Temperature:4 °C
Specifications
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