Specifications
- Assay duration:Multiple steps
- Assay Type (ELISA with LOV):Sandwich
- Format:Pre-coated
- Primary antibody reactivity:Other
- Target protein:FGF23
- Description:Porcine FGF23 ELISA kit
- Size:96 tests
- Sample Type:Serum, plasma, tissue homogenates and other biological fluids
- Cross Reactivity:Porcine FGF23 ELISA kit exhibits high specificity and excellent specificity for the detection of porcine FGF23. No significant cross-reactivity or interference between FGF23 and analogues was observed.
- Detection Method:Colorimetric
- Time to Results:4 h 30 min
- Assay Principle:Quantitative
- Shelf Life:Store for 6 months at 4 °C
- Detection Range:62.5 - 4000 pg/ml
- Storage Temperature:4 °C
- Sample Volume:100 μl
- Sensitivity:37.5 pg/ml
- Regulatory Status:RUO
- Cat. No.:76738-152
Specifications
About this item
Porcine FGF23 ELISA kit is a sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of porcine FGF23 in serum, plasma, tissue homogenates, and other biological fluids.
- Ready-To-Use ELISA Kit
- Detection Range: 62.5 - 4000 pg/ml
- Sensitivity: 37.5 pg/ml
- Sample Type: Serum, plasma, tissue homogenates, and other biological fluids
- Assay Precision: Intra-Assay: CV <8%, Inter-Assay: CV <10%
Porcine FGF23 ELISA kit (A74396) employs the sandwich enzyme immunoassay technique for the quantitative measurement of porcine FGF23 in serum, plasma, tissue homogenates, and other biological fluids. An antibody specific for FGF23 has been pre-coated onto a 96-well microtiter plate. The standards and test samples are added into the wells and the FGF23 present in each sample is bound to the wells by the immobilized antibody. Following incubation, the wells are washed and then incubated with Biotinylated Anti-FGF23 Antibody, which binds the captured FGF23 present in each well. Following incubation, unbound biotinylated detection antibody is removed by washing, and an HRP-Streptavidin conjugate is added to the wells and the microtiter plate is incubated. Following incubation and washing, TMB substrate solution is then used to visualize the HRP enzymatic reaction by catalysis to produce a blue-coloured product that changes to yellow after addition of acidic stop solution. The density of yellow is proportional to the amount of FGF23 captured in each well. The concentration of FGF23 can then be calculated by reading the O.D. absorbance at 450 nm in a microplate reader and referring to the standard curve.