Specifications
- Assay duration:Multiple steps
- Assay Type (ELISA with LOV):Sandwich
- Format:Pre-coated
- Primary antibody reactivity:Human
- Target protein:S100A9
- Description:Human S100A9 ELISA kit
- Size:96 tests
- Sample Type:Serum, plasma, tissue homogenates and other biological fluids
- Cross Reactivity:Human S100A9 ELISA kit exhibits high specificity and excellent specificity for the detection of human S100A9. No significant cross-reactivity or interference between S100A9 and analogues was observed.
- Detection Method:Colorimetric
- Time to Results:4 h 30 min
- Assay Principle:Quantitative
- Shelf Life:Store for 6 months at 4 °C
- Detection Range:0.781 - 50 ng/ml
- Storage Temperature:4 °C
- Sample Volume:100 μl
- Sensitivity:0.469 ng/ml
- Regulatory Status:RUO
- Cat. No.:76732-372
Specifications
About this item
Human S100A9 ELISA kit is a sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of human S100A9 in serum, plasma, tissue homogenates, and other biological fluids.
- Ready to use ELISA kit
- Detection range: 0.781 to 50 ng/ml
- Sensitivity: 0.469 ng/ml
- Sample type: Serum. plasma. tissue homogenates. and other biological fluids
- Assay precision: Intra assay: CV <8%. Inter assay: CV <10%
Human S100A9 ELISA kit (A75801) employs the sandwich enzyme immunoassay technique for the quantitative measurement of human S100A9 in serum, plasma, tissue homogenates, and other biological fluids. An antibody specific for S100A9 has been pre-coated onto a 96-well microtiter plate. The standards and test samples are added into the wells and the S100A9 present in each sample is bound to the wells by the immobilized antibody. Following incubation, the wells are washed and then incubated with Biotinylated Anti-S100A9 Antibody, which binds the captured S100A9 present in each well. Following incubation, unbound biotinylated detection antibody is removed by washing, and an HRP-Streptavidin conjugate is added to the wells and the microtiter plate is incubated. Following incubation and washing, TMB substrate solution is then used to visualize the HRP enzymatic reaction by catalysis to produce a blue-coloured product that changes to yellow after addition of acidic stop solution. The density of yellow is proportional to the amount of S100A9 captured in each well. The concentration of S100A9 can then be calculated by reading the O.D. absorbance at 450 nm in a microplate reader and referring to the standard curve.