Specifications
- Assay duration:Multiple steps
- Assay Type (ELISA with LOV):Sandwich
- Format:Pre-coated
- Primary antibody reactivity:Human
- Target protein:HSP90 alpha
- Description:Human HSP90 alpha ELISA kit
- Size:96 tests
- Sample Type:Serum, plasma, tissue homogenates and other biological fluids
- Cross Reactivity:Human HSP90 alpha ELISA kit exhibits high specificity and excellent specificity for the detection of human HSP90 alpha. No significant cross-reactivity or interference between HSP90 alpha and analogues was observed.
- Detection Method:Colorimetric
- Time to Results:4 h 30 min
- Assay Principle:Quantitative
- Shelf Life:Store for 6 months at 4 °C
- Detection Range:3.125 - 200 ng/ml
- Storage Temperature:4 °C
- Sample Volume:100 μl
- Sensitivity:1.875 ng/ml
- Regulatory Status:RUO
- Cat. No.:76732-132
Specifications
About this item
Human HSP90 alpha ELISA kit is a sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of human HSP90 alpha in serum, plasma, tissue homogenates, and other biological fluids.
- Ready-To-Use ELISA Kit
- Detection Range: 3.125 - 200 ng/ml
- Sensitivity: 1.875 ng/ml
- Sample Type: Serum, plasma, tissue homogenates, and other biological fluids
- Assay Precision: Intra-Assay: CV <8%, Inter-Assay: CV <10%
Human HSP90 alpha ELISA kit (A74297) employs the sandwich enzyme immunoassay technique for the quantitative measurement of human HSP90 alpha in serum, plasma, tissue homogenates, and other biological fluids. An antibody specific for HSP90 alpha has been pre-coated onto a 96-well microtiter plate. The standards and test samples are added into the wells and the HSP90 alpha present in each sample is bound to the wells by the immobilized antibody. Following incubation, the wells are washed and then incubated with Biotinylated Anti-HSP90 alpha Antibody, which binds the captured HSP90 alpha present in each well. Following incubation, unbound biotinylated detection antibody is removed by washing, and an HRP-Streptavidin conjugate is added to the wells and the microtiter plate is incubated. Following incubation and washing, TMB substrate solution is then used to visualize the HRP enzymatic reaction by catalysis to produce a blue-coloured product that changes to yellow after addition of acidic stop solution. The density of yellow is proportional to the amount of HSP90 alpha captured in each well. The concentration of HSP90 alpha can then be calculated by reading the O.D. absorbance at 450 nm in a microplate reader and referring to the standard curve.