- Assay duration:Multiple steps
- Assay Type (ELISA with LOV):Sandwich
- Format:Pre-coated
- Primary antibody reactivity:Monkey
- Target protein:MIP3 alpha
- Description:Monkey MIP3 alpha ELISA kit
- Size:96 tests
- Sample Type:Serum, plasma, tissue homogenates and other biological fluids
- Cross Reactivity:Monkey MIP3 alpha ELISA kit exhibits high specificity and excellent specificity for the detection of monkey MIP3 alpha. No significant cross-reactivity or interference between MIP3 alpha and analogues was observed.
- Detection Method:Colorimetric
- Time to Results:4 h 30 min
- Assay Principle:Quantitative
- Shelf Life:Store for 6 months at 4 °C
- Detection Range:31.25 - 2000 pg/ml
- Storage Temperature:4 °C
- Sample Volume:100 μl
- Sensitivity:18.75 pg/ml
- Regulatory Status:RUO
- Cat. No.:76731-392
Monkey MIP3 alpha ELISA kit is a sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of monkey MIP3 alpha in serum, plasma, tissue homogenates, and other biological fluids.
- Ready-To-Use ELISA Kit
- Detection Range: 31.25 - 2000 pg/ml
- Sensitivity: 18.75 pg/ml
- Sample Type: Serum, plasma, tissue homogenates, and other biological fluids
- Assay Precision: Intra-Assay: CV <8%, Inter-Assay: CV <10%
Monkey MIP3 alpha ELISA kit (A74225) employs the sandwich enzyme immunoassay technique for the quantitative measurement of monkey MIP3 alpha in serum, plasma, tissue homogenates, and other biological fluids. An antibody specific for MIP3 alpha has been pre-coated onto a 96-well microtiter plate. The standards and test samples are added into the wells and the MIP3 alpha present in each sample is bound to the wells by the immobilized antibody. Following incubation, the wells are washed and then incubated with Biotinylated Anti-MIP3 alpha Antibody, which binds the captured MIP3 alpha present in each well. Following incubation, unbound biotinylated detection antibody is removed by washing, and an HRP-Streptavidin conjugate is added to the wells and the microtiter plate is incubated. Following incubation and washing, TMB substrate solution is then used to visualize the HRP enzymatic reaction by catalysis to produce a blue-coloured product that changes to yellow after addition of acidic stop solution. The density of yellow is proportional to the amount of MIP3 alpha captured in each well. The concentration of MIP3 alpha can then be calculated by reading the O.D. absorbance at 450 nm in a microplate reader and referring to the standard curve.