Rat GSTA4 ELISA kit is a competitive Enzyme-Linked Immunosorbent Assay (cELISA) designed for the in vitro quantitative determination of rat GSTA4 in serum, plasma, tissue homogenates, and other biological fluids.

• Ready To Use ELISA Kit
• Detection Range: 0.781 to 50 ng/ml
• Sensitivity: 0.469 ng/ml
• Sample Type: Serum, plasma, tissue homogenates, and other biological fluids
• Assay Precision: Intra - Assay: CV <8%, Inter - Assay: CV <10%

Rat GSTA4 ELISA Kit (A79410) employs the competitive enzyme immunoassay technique for the quantitative measurement of rat GSTA4 in serum, plasma, tissue homogenates, and other biological fluids. The 96-well microtiter plate has been pre-coated with GSTA4 antigen. During the incubation, GSTA4 present in the samples or standards competes with the fixed amount of immobilized GSTA4 for binding sites on the Biotinylated Anti-GSTA4 Antibody. The more GSTA4 present in a sample or standard, the less Biotinylated Anti-GSTA4 Antibody that binds to the plate. Following incubation, unbound Biotinylated Anti-GSTA4 Antibody is removed by washing, and an HRP-Streptavidin conjugate is added to the wells and the microtiter plate is incubated. Following incubation and washing, TMB substrate solution is then used to visualize the HRP enzymatic reaction by catalysis to produce a blue-coloured product that changes to yellow after addition of acidic stop solution. The density of yellow is inversely proportional to the amount of GSTA4 present in each sample or standard. The concentration of GSTA4 can then be calculated by reading the O.D. absorbance at 450nm in a microplate reader and referring to the standard curve.