Specifications
- Assay duration:Multiple steps
- Assay Type (ELISA with LOV):Competitive
- Format:Pre-coated
- Primary antibody reactivity:Other
- Target protein:25-Hydroxy Vitamin D2
- Description:25-Hydroxy vitamin D2 ELISA kit
- Size:96 tests
- Sample Type:Serum, plasma, tissue homogenates, and other biological fluids
- Cross Reactivity:25-Hydroxy Vitamin D2 ELISA Kit exhibits high specificity and excellent specificity for the detection of universal 25-Hydroxy Vitamin D2. No significant cross-reactivity or interference between 25-Hydroxy Vitamin D2 and analogues was observed.
- Detection Method:Colorimetric
- Time to Results:4 h 30 min
- Assay Principle:Quantitative
- Shelf Life:Store for 6 months at 4 °C
- Detection Range:0.781 - 50 ng/ml
- Storage Temperature:4 °C
- Sample Volume:50 μl
- Sensitivity:0.469 ng/ml
- Regulatory Status:RUO
- Cat. No.:76744-724
Specifications
About this item
25-Hydroxy vitamin D2 ELISA kit is a competitive Enzyme-Linked Immunosorbent Assay (cELISA) designed for the in vitro quantitative determination of 25-hydroxy vitamin D2 in serum, plasma, tissue homogenates, and other biological fluids.
- Ready-to-use ELISA kit
- Assay precision: Intra-assay: CV <8%, Inter-assay: CV <10%
25-Hydroxy vitamin D2 ELISA kit (A303875) employs the competitive enzyme immunoassay technique for the quantitative measurement of universal 25-Hydroxy vitamin D2 in serum, plasma, tissue homogenates, and other biological fluids. The 96-well microtiter plate has been pre-coated with 25-Hydroxy vitamin D2 antigen. During the incubation, 25-Hydroxy vitamin D2 present in the samples or standards competes with the fixed amount of immobilized 25-Hydroxy vitamin D2 for binding sites on the Biotinylated anti-25-Hydroxy vitamin D2 antibody. The more 25-Hydroxy vitamin D2 present in a sample or standard, the less Biotinylated anti-25-Hydroxy vitamin D2 antibody that binds to the plate. Following incubation, unbound Biotinylated anti-25-Hydroxy vitamin D2 antibody is removed by washing, and an HRP-Streptavidin conjugate is added to the wells and the microtiter plate is incubated. Following incubation and washing, TMB substrate solution is then used to visualize the HRP enzymatic reaction by catalysis to produce a blue-coloured product that changes to yellow after addition of acidic stop solution. The density of yellow is inversely proportional to the amount of 25-Hydroxy vitamin D2 present in each sample or standard. The concentration of 25-Hydroxy vitamin D2 can then be calculated by reading the O.D. absorbance at 450 nm in a microplate reader and referring to the standard curve.