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Human Complement C1s ELISA Kit
Human Complement C1s ELISA Kit
  76743-204
 :  ANTIBODIES.COM LLC
 :  
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Human Complement C1s ELISA Kit
  76743-204
 :  ANTIBODIES.COM LLC
 :  A79151-96
 :  

 

  • Assay duration:
    Multiple steps
  • Assay Type (ELISA with LOV):
    Sandwich
  • Format:
    Pre-coated
  • Primary antibody reactivity:
    Human
  • Target protein:
    Complement C1s
  • Description:
    Human complement C1s ELISA kit
  • Size:
    96 tests
  • Sample Type:
    Serum, plasma, tissue homogenates and other biological fluids
  • Cross Reactivity:
    Human Complement C1s ELISA kit exhibits high specificity and excellent specificity for the detection of human Complement C1s. No significant cross-reactivity or interference between Complement C1s and analogues was observed.
  • Detection Method:
    Colorimetric
  • Time to Results:
    4 h 30 min
  • Assay Principle:
    Quantitative
  • Shelf Life:
    Store for 6 months at 4 °C
  • Detection Range:
    1.563 - 100 ng/ml
  • Storage Temperature:
    4 °C
  • Sample Volume:
    100 μl
  • Sensitivity:
    0.938 ng/ml
  • Regulatory Status:
    RUO
  • Cat. No.:
    76743-204

 

 

Human Complement C1s ELISA kit is a sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of human Complement C1s in serum, plasma, tissue homogenates, and other biological fluids.

  • Ready-To-Use ELISA Kit
  • Detection Range: 1.563 - 100 ng/ml
  • Sensitivity: 0.938 ng/ml
  • Sample Type: Serum, plasma, tissue homogenates, and other biological fluids
  • Assay Precision: Intra-Assay: CV <8%, Inter-Assay: CV <10%

Human Complement C1s ELISA kit (A79151) employs the sandwich enzyme immunoassay technique for the quantitative measurement of human Complement C1s in serum, plasma, tissue homogenates, and other biological fluids. An antibody specific for Complement C1s has been pre-coated onto a 96-well microtiter plate. The standards and test samples are added into the wells and the Complement C1s present in each sample is bound to the wells by the immobilized antibody. Following incubation, the wells are washed and then incubated with Biotinylated Anti-Complement C1s Antibody, which binds the captured Complement C1s present in each well. Following incubation, unbound biotinylated detection antibody is removed by washing, and an HRP-Streptavidin conjugate is added to the wells and the microtiter plate is incubated. Following incubation and washing, TMB substrate solution is then used to visualize the HRP enzymatic reaction by catalysis to produce a blue-coloured product that changes to yellow after addition of acidic stop solution. The density of yellow is proportional to the amount of Complement C1s captured in each well. The concentration of Complement C1s can then be calculated by reading the O.D. absorbance at 450 nm in a microplate reader and referring to the standard curve.

 : This is a ready-to-use ELISA kit that contains: Pre-coated 96 well microplate, lyophilized standard, sample dilution buffer, biotinylated detection antibody (100X), antibody dilution buffer, HRP-Streptavidin Conjugate (SABC) (100X), SABC dilution buffer, TMB substrate, stop solution, wash buffer (25X), plate sealers, foil pouch, and protocol manual.